Difference between revisions of "Part:BBa K2916027"

 
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__NOTOC__
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<partinfo>BBa_K2916026 short</partinfo>
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<partinfo>BBa_K2916027 short</partinfo>
  
 
This part is used for expression of PheRS needed for the PURE and OnePot PURE cell-free systems.  
 
This part is used for expression of PheRS needed for the PURE and OnePot PURE cell-free systems.  
  
  
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<!-- Add more about the biology of this part here
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<!-- Add more about the biology of this part here-->
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===Usage and Biology===
 
===Usage and Biology===
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Used in OnePot PURE
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2916026 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2916027 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2916026 parameters</partinfo>
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<partinfo>BBa_K2916027 parameters</partinfo>
 
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Revision as of 17:36, 25 September 2019


Expression of PheRS in E.coli

This part is used for expression of PheRS needed for the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2533
    Illegal BamHI site found at 140
    Illegal BamHI site found at 1881
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1271
    Illegal AgeI site found at 1264
    Illegal AgeI site found at 2186
    Illegal AgeI site found at 3038
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2984
    Illegal SapI.rc site found at 307
    Illegal SapI.rc site found at 2960