Difference between revisions of "Part:BBa K2927022"
| Line 3: | Line 3: | ||
We have made a change on 5 nucleotides of crRNA 3 from 5’ end to make the mutated crRNA 3. It is the sequence for testing whether our purified Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 3 is too short to be the functional protein. Thus, there is no risk for the spread out of ASFV from our project. | We have made a change on 5 nucleotides of crRNA 3 from 5’ end to make the mutated crRNA 3. It is the sequence for testing whether our purified Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 3 is too short to be the functional protein. Thus, there is no risk for the spread out of ASFV from our project. | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 06:27, 2 October 2019
Negative control sequence for crRNA 3 target
We have made a change on 5 nucleotides of crRNA 3 from 5’ end to make the mutated crRNA 3. It is the sequence for testing whether our purified Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 3 is too short to be the functional protein. Thus, there is no risk for the spread out of ASFV from our project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]