Difference between revisions of "Part:BBa K2927020"
 
Line 2: Line 2:
 
<partinfo>BBa_K2927020 short</partinfo>
 
<partinfo>BBa_K2927020 short</partinfo>
  
−
We have made a change on 5 nucleotides of crRNA 1 from 5’ end to make the mutated crRNA 1. It is the sequence for testing whether our purified Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 1 is too short to be the functional protein. Thus, there is no risk for the spread out of ASFV from our project.
+
To generate negative control vector that cannot bind by crRNA-1, we mutated five nucleotides within the target region of crRNA-1 in positive control vector. Negative control vector is applied to examine the specify of Cas12a activation.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 05:59, 16 October 2019

Negative control sequence for crRNA 1 target

To generate negative control vector that cannot bind by crRNA-1, we mutated five nucleotides within the target region of crRNA-1 in positive control vector. Negative control vector is applied to examine the specify of Cas12a activation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]