Difference between revisions of "Part:BBa K2927011"

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crRNA is crucial for the CRISPR Cas system. It is the short sequence that combines with Cas12a protein and forms a functional complex. Once crRNA binds with Cas12a protein, the complex capable to target and cleave targeted double-stranded DNA sequence (cis cleavage activity) and the cleavage of double-stranded DNA activates the cleavage of non-specific ssDNA (trans cleavage activity). In our project, we use crRNA for detecting a part sequence of vp72 membrane protein of African swine fever virus (ASFV).  
 
crRNA is crucial for the CRISPR Cas system. It is the short sequence that combines with Cas12a protein and forms a functional complex. Once crRNA binds with Cas12a protein, the complex capable to target and cleave targeted double-stranded DNA sequence (cis cleavage activity) and the cleavage of double-stranded DNA activates the cleavage of non-specific ssDNA (trans cleavage activity). In our project, we use crRNA for detecting a part sequence of vp72 membrane protein of African swine fever virus (ASFV).  
  
When crRNA 2 binds with Cas12a protein, the protein may carry out cis-trans cleavage activity once it recognizes its target. By the way, though crRNA 2 is derived from ASFV, there is no possibility for virulence. The sequence of crRNA 2 is derived from p72 which is the capsid of ASFV. Therefore, the sequence of crRNA 2 is too short to be a functional protein. Thus, there is probably no risk for the spread out of ASFV from our project.
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When crRNA 2 binds with Cas12a protein, the protein may carry out cis-trans cleavage activity once it recognizes its target. By the way, though crRNA 2 is derived from ASFV, there is no possibility for virulence. The sequence of crRNA 2 is derived from p72 which is the capsid of ASFV. Therefore, the sequence of crRNA 2 is too short to be a functional protein. Thus, there is probably no risk for the spread out of ASFV from our project. Therefore, the design of this gene expression was reported in the check-in form and got approved.
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Revision as of 16:02, 1 October 2019


DNA template of crRNA 2 for LbCas12a

crRNA is crucial for the CRISPR Cas system. It is the short sequence that combines with Cas12a protein and forms a functional complex. Once crRNA binds with Cas12a protein, the complex capable to target and cleave targeted double-stranded DNA sequence (cis cleavage activity) and the cleavage of double-stranded DNA activates the cleavage of non-specific ssDNA (trans cleavage activity). In our project, we use crRNA for detecting a part sequence of vp72 membrane protein of African swine fever virus (ASFV).

When crRNA 2 binds with Cas12a protein, the protein may carry out cis-trans cleavage activity once it recognizes its target. By the way, though crRNA 2 is derived from ASFV, there is no possibility for virulence. The sequence of crRNA 2 is derived from p72 which is the capsid of ASFV. Therefore, the sequence of crRNA 2 is too short to be a functional protein. Thus, there is probably no risk for the spread out of ASFV from our project. Therefore, the design of this gene expression was reported in the check-in form and got approved.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]