Difference between revisions of "Part:BBa K3187020"
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The Sortase A7M is a mutated form of the enzyme Sortase from Staphylococcus aureus. Sortase is an enzyme that catalyzes the formation of a peptide bond between a protein containing a C-terminal LPETG amino acid sequence and a protein containing an N-terminal polyG sequence (two or more glycines on the N- terminus). Peptide bond formation is between the threonine (T) of the LPETG and the N- terminal glycine. Due to the mutations it is calcium-independent which is why it can be used in vivo without disturbing the calcium concentrations in cells. It has a molecular weight of 17,85 kDa. | The Sortase A7M is a mutated form of the enzyme Sortase from Staphylococcus aureus. Sortase is an enzyme that catalyzes the formation of a peptide bond between a protein containing a C-terminal LPETG amino acid sequence and a protein containing an N-terminal polyG sequence (two or more glycines on the N- terminus). Peptide bond formation is between the threonine (T) of the LPETG and the N- terminal glycine. Due to the mutations it is calcium-independent which is why it can be used in vivo without disturbing the calcium concentrations in cells. It has a molecular weight of 17,85 kDa. | ||
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| + | <p style="text-align:center;"><img src="https://static.igem.org/mediawiki/2018/c/c7/T--TU_Darmstadt--NADPHassay.png" alt="ycdW mechanism“ width="150" height="180"></p> | ||
| + | <p align="center"><b>Figure 2:</b> Reaction mechanism of YcdW </p> <br> | ||
| + | </html> | ||
Revision as of 12:52, 24 September 2019
Tobacco Vein Mottling Virus Protease Cleavage Site
The Sortase A7M is a mutated form of the enzyme Sortase from Staphylococcus aureus. Sortase is an enzyme that catalyzes the formation of a peptide bond between a protein containing a C-terminal LPETG amino acid sequence and a protein containing an N-terminal polyG sequence (two or more glycines on the N- terminus). Peptide bond formation is between the threonine (T) of the LPETG and the N- terminal glycine. Due to the mutations it is calcium-independent which is why it can be used in vivo without disturbing the calcium concentrations in cells. It has a molecular weight of 17,85 kDa.
Figure 2: Reaction mechanism of YcdW
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]