Difference between revisions of "Part:BBa K581005"

Revision as of 05:13, 23 September 2019

Pc+sgrS(wt)+Terminator (small RNA regulator, conjugate part of ptsG(wt))

SgrS(sugar transport-related sRNA)(wt) is a small RNA regulator that help cells recover from glucose-phosphate stress by base pairing with ptsG(wt)[1] mRNA. SgrS regulates ptsG mRNA by short, imperfect base-pairing interactions; as a result, the expression of PtsG is repressed.

Teppei Morita et.al’ s work suggests that two mutations (C85G and C87G) in ptsG mRNA could completely impair the ability of SgrS to downregulate its expression, while compensatory mutations of SgrS (G178C and G176C) restore the gene silencing ability. These results indicate that it is the base pairing of the two RNAs rather than particular nucleotides that is important for SgrS action. They have also illustrated that sequence outside this region, even though complementary, is rather dispensable for the efficient silencing (Kawamoto et al., 2006). This makes mutant ptsG/SgrS pairs orthogonal to genetic context of the host cell. Therefore we choose this couple of conjugate mRNA/sRNA as the foundation of our comparator device design.

SgrS(wt) in this part will be constitutively expressed in E.coli after transformation processing.

                 
                Fig.1 Sequence alignment of wildtype ptsG/SgrS pair and its mutant complementary pairs. 

Sequence and Features

Group

NUS_Singapore 2019

Author

Sheena Chan

Summary and Uploads

Link to our Improved Part

https://parts.igem.org/Part:BBa_K3198005