Difference between revisions of "Part:BBa K2957007"
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<partinfo>BBa_K2957099 short</partinfo>
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<partinfo>BBa_K2957007 short</partinfo>
  
 
Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance
 
Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance
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What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.  
 
What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.  
  
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How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
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How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2957099 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2957007 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2957099 parameters</partinfo>
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<partinfo>BBa_K2957007 parameters</partinfo>
 
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Revision as of 22:53, 19 October 2019


hEF1a, IL-8 (CXCL-8), NeonGreen Tag

Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1256
    Illegal PstI site found at 699
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 699
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 473
    Illegal BamHI site found at 715
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1256
    Illegal PstI site found at 699
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1256
    Illegal PstI site found at 699
    Illegal AgeI site found at 81
    Illegal AgeI site found at 1262
  • 1000
    COMPATIBLE WITH RFC[1000]