Difference between revisions of "Part:BBa K2926003"
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==Characterisation== | ==Characterisation== | ||
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− | We followed the standard <a href="https://www.protocols.io/view/calibration-protocol-plate-reader-fluorescence-cal-6zrhf56">iGEM protocoll for GFP fluorescence calibration</a>, | + | We followed the standard <a href="https://www.protocols.io/view/calibration-protocol-plate-reader-fluorescence-cal-6zrhf56">iGEM protocoll for GFP fluorescence calibration</a>, measuring our cultures against Flourescin. |
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We performed the analysis with <i>E. coli DH5α</i> as well as <i>S. cerevisiae</i> cultures. | We performed the analysis with <i>E. coli DH5α</i> as well as <i>S. cerevisiae</i> cultures. | ||
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− | + | <b>Please see <a href="XXX">BBa_K2926073</a> for the characterization results of this part.</b> | |
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Revision as of 21:05, 22 September 2019
GALL yeast promoter
Usage and Biology
GALL is a shortend version of the natural GAl1 promotor from Saccharomyces cerevisiae, which is regulating the gene encoding the galactokinase. The GAL1 promotor is tightly repressed by glucose and strongly induced by galactose. While the GAL1 Promotor is composed of the 461bp upstream of the gal1 gene, GALL is shortend by 31bt to 430bp upstream of the gene. Thats because the GALL Promotor is lacking one of the three upstream activating sequences, which are required for full induction by galactose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 47
- 1000COMPATIBLE WITH RFC[1000]
Contents
Plasmid Design
For the analysis and characterization of the GALL Promotor XXXX in combiantion with the TPs1 Terminaror XXXX, mCherry XXXX was cloned beteen them using Gibson Assembly. The GALL Promotor and TPs1 Terminator where optained as a gBlock from IDT.
Sequencing Results
The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.
Characterisation
We followed the standard iGEM protocoll for GFP fluorescence calibration, measuring our cultures against Flourescin.
We performed the analysis with E. coli DH5α as well as S. cerevisiae cultures.
Please see BBa_K2926073 for the characterization results of this part.
References