Difference between revisions of "Part:BBa K137055"
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<partinfo>BBa_K137055 short</partinfo>
 
<partinfo>BBa_K137055 short</partinfo>
  
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''pabA'' is one of three genes (''pabA'', ''pabB'', ''pabC'') involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter J23100 and the strong RBS B0034, and in front of the terminator B0015. Currently it is in the inducible copy plasmid pSB2K3, which can be induced to high copy via IPTG.   
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''pabA'' is one of three genes (''pabA'', ''pabB'', ''pabC'') involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter J23100 and the strong RBS B0034, and in front of the terminator B0015. Currently it is in the high copy plasmid pSB1AK3.   
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/PABA_HPLC_assay PABA HPLC protocol], K137055 in pSB1AK3 in DH10B produced 700ng/mL of PABA (vs. negligible production for DH10B without K137055).
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[[Image:PABA.png|700px]]
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<div style="clear:both;"></div>
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HPLC trace. PABA elutes at 4.5 minutes. Blue: DH10B (negative control), Green: K137055
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K137055 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K137055 SequenceAndFeatures</partinfo>

Revision as of 17:15, 17 October 2008

Constitutive pabA expression construct

pabA is one of three genes (pabA, pabB, pabC) involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter J23100 and the strong RBS B0034, and in front of the terminator B0015. Currently it is in the high copy plasmid pSB1AK3.


Usage and Biology

When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/PABA_HPLC_assay PABA HPLC protocol], K137055 in pSB1AK3 in DH10B produced 700ng/mL of PABA (vs. negligible production for DH10B without K137055).

PABA.png

HPLC trace. PABA elutes at 4.5 minutes. Blue: DH10B (negative control), Green: K137055

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]