Difference between revisions of "Part:BBa K3182300"
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<partinfo>BBa_K3182300 short</partinfo> | <partinfo>BBa_K3182300 short</partinfo> | ||
− | This is a improved variant of BBa_K2671000. | + | This is a improved variant of BBa_K2671000. The biobrick codes for mNeonGreen (mNG), which is a fluorescent protein with great intensity. The protein is currently ranked third in intensity, only beaten by mVenus-Q69M (basic) and Skylan-S (photoswitchable). |
+ | |||
+ | <br><br> | ||
+ | This part has a T7-system as well as a 5’-UTR region, instead of the AraC-pBAD system present in the non-improved biobrick BBa_K2671000. By using the T7-DNA-directed-RNA-polymerase (T7-DdRNAP) from a bacteriophage over the native DNA-directed-RNA-polymerase (n-DdRNAP) native in E. coli the expression is greatly increased. This part requires a host carrying the T7-DdRNAP, such as E. coli BL21 (DE3) Gold which was the chassi we used. | ||
Revision as of 12:11, 20 September 2019
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 91
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Introduction
pT7-mNG
This is a improved variant of BBa_K2671000. The biobrick codes for mNeonGreen (mNG), which is a fluorescent protein with great intensity. The protein is currently ranked third in intensity, only beaten by mVenus-Q69M (basic) and Skylan-S (photoswitchable).
This part has a T7-system as well as a 5’-UTR region, instead of the AraC-pBAD system present in the non-improved biobrick BBa_K2671000. By using the T7-DNA-directed-RNA-polymerase (T7-DdRNAP) from a bacteriophage over the native DNA-directed-RNA-polymerase (n-DdRNAP) native in E. coli the expression is greatly increased. This part requires a host carrying the T7-DdRNAP, such as E. coli BL21 (DE3) Gold which was the chassi we used.
Usage and Biology