Difference between revisions of "Part:BBa K2963032:Design"

 
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===Design Notes===
 
===Design Notes===
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We use the "Ptac-lacO-rbs-racE-T7t" part trying to make the expression of racE inducible.  
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The strength of racE gene expression affects the proportion of D/L glutamate monomer in γ-PGA. In our project, we wanted to regulate the expression of the racE gene to produce γ-PGA with different D/L-glutamate monomer ratios.  
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Tac Promoter contains a kind of operator gene called lacO. We changed the number of operator gene in tac promoter assembling other basic parts to form our composite parts: BBa_K2963032 and BBa_K2963033. The experiment process please visit our EXPERIMENT page.
  
 
===Source===
 
===Source===

Revision as of 15:11, 13 October 2019


racE- encoding racemase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The strength of racE gene expression affects the proportion of D/L glutamate monomer in γ-PGA. In our project, we wanted to regulate the expression of the racE gene to produce γ-PGA with different D/L-glutamate monomer ratios. Tac Promoter contains a kind of operator gene called lacO. We changed the number of operator gene in tac promoter assembling other basic parts to form our composite parts: BBa_K2963032 and BBa_K2963033. The experiment process please visit our EXPERIMENT page.

Source

racE from Bacillus Genome.

References