Difference between revisions of "Part:BBa K2951004"
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<partinfo>BBa_K2951004 short</partinfo> | <partinfo>BBa_K2951004 short</partinfo> | ||
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+ | </p> | ||
+ | </html> | ||
+ | __TOC__ | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part is a ssDNA aptamer that targets the nucleocapsid protein of influenza A virus (A/Michigan/297/2017(H1N1)).(sequence from NCBI(CY261696.1) gene:34-1530) | ||
+ | The probe can be used for on-site influenza detection. | ||
+ | |||
+ | ===Characterization=== | ||
+ | ====Titer Value==== | ||
+ | The titer value of this aptamer towards its target protein nucleocaspid is determined by practicing two types of ELISA, according to the coated protein, two important factors are tested: | ||
+ | {| | ||
+ | ! style="text-align:left;"| Factor | ||
+ | ! style="text-align:left;" | Method | ||
+ | |- | ||
+ | |Affinity | ||
+ | |Non-competitive ELISA by coating target protein | ||
+ | |- | ||
+ | |Specificity | ||
+ | |competitive ELISA by coating un-target protein | ||
+ | |- | ||
+ | |} | ||
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Revision as of 17:30, 19 October 2019
Apt-influenzaA-NP
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]