Difference between revisions of "Part:BBa K3111002:Design"

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===Source===
 
===Source===
  
−
Enterokinase site is taken from Novagen plasmid pET-51b
+
Enterokinase site is taken from Novagen plasmid pET-51b. Stop codon removed at the end of BBa_K2686001 BioBrick as a result of fusion at the C-terminus of the protein.
  
 
===References===
 
===References===

Revision as of 20:30, 18 September 2019


T. maritima encapsulin from BBa_K2686001 (no stop codon)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 375
    Illegal SapI.rc site found at 406


Design Notes

-


Source

Enterokinase site is taken from Novagen plasmid pET-51b. Stop codon removed at the end of BBa_K2686001 BioBrick as a result of fusion at the C-terminus of the protein.

References