Difference between revisions of "Part:BBa K3182300"
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===Usage and Biology===
 
  
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<h1>Usage and Biology</h1>
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[[File:T--Linkoping_Sweden--mgnen.png|420px|thumb|left|<b>Figure 2.</b> ]]
  
  

Revision as of 10:03, 20 September 2019

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 91
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

pT7-mNG

This is a improved variant of BBa_K2671000. This part codes for mNeonGreen, which is a fluorescent protein with great intensity. mNeonGreen has a excitation maximum at 506 nm and a emission maximum at 517, providing a small Stoke's shift. It's color can be seen under normal white light as a yellow-green color. This part includes a T7 promotor as well as a 5'-UTR region for higher expression.


Figure 1. A schematic representation of the improvement. Where the promotor (small green arrow) has been changed from pBAD --> pT7. Extra bases which are non-coding (black line behind mNeonGreen in upper part) was also removed.