Difference between revisions of "Part:BBa K3128010"

Revision as of 08:22, 23 September 2019

Flexible Glycine Glycine Serine (GGS) Linker (54 aa)

Usage and Biology

Designing fusion proteins is sometimes tricky because of potential misfolding and protein steric hindrance. To avoid these problems, linkers are often used. They can be either rigid or flexible. Flexible linkers are used to enable a certain degree of movements to the different parts of the newly generated fusion protein. Moreover, they allow to generate fusion proteins without interfering with the function of each domain. They are constituted with a succession of Gly and Ser residues. This linker has the following formula: (Gly-Gly-Ser)_n , where n = 54. These sequences can be repeated in order to obtain an appropriate length separation of functional domains and can also be shortened to maintain inter-domain reactions.

In the iGEM Grenoble 2019 project, these linkers are used in order to gain a certain flexibility with the adenylate subparts fused to the OmpX proteins. Thus allowing the utilization of a bacterial two-hybrid system in the external membrane of the bacteria.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-Designing fusion proteins is sometimes tricky because of potential misfolding and... To avoid these problems, linkers are often used. Flexible linkers to enable a certain degree of movements of the fusion protein different parts. They are constituted with a succession of Gly and Ser residues. This linker has the following formula: (Gly-Gly-Ser)n, where n = 54 here.
+Designing fusion proteins is sometimes tricky because of potential misfolding and protein steric hindrance. To avoid these problems, linkers are often used. They can be either rigid or flexible.
-Sequences can be repeated in order to obtain an appropriate length separation of functional domains and can also be shortened to maintain inter-domain reactions.
+Flexible linkers are used to enable a certain degree of movements to the different parts of the newly generated fusion protein. Moreover, they allow to generate fusion proteins without interfering with the function of each domain.
-In the iGEM Grenoble 2019 project, these linkers are used in order to have certain flexibility of the adenylate subparts fused to the OmpX proteins. Thus allowing the utilization of a bacterial two-hybrid system in the external membrane of the bacteria.
+They are constituted with a succession of Gly and Ser residues. This linker has the following formula: (Gly-Gly-Ser)<sub>n</sub> , where n = 54.
+These sequences can be repeated in order to obtain an appropriate length separation of functional domains and can also be shortened to maintain inter-domain reactions.
+In the iGEM Grenoble 2019 project, these linkers are used in order to gain a certain flexibility with the adenylate subparts fused to the OmpX proteins. Thus allowing the utilization of a bacterial two-hybrid system in the external membrane of the bacteria.