Difference between revisions of "Part:BBa K2996703"

 
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RpoA encodes the DNA-directed RNA polymerase subunit alpha, which  plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme. It may activate transcription more effectively, compared to RpoZ used in other expereiments.
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This RpoA-dCas9 fusion protein should be an inovated transcription activator.
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Cas1 and Cas2 are the only Cas proteins required for genetic recording of infections through spacer acquisition from invader DNA, thus they are called adaptation complex. In adaptation stage, short DNA fragments generated from phage DNA or other foreign DNA are captured by the adaptation complex. Following trimming of excess DNA, the adaptation complex integrates these fragments into the CRISPR array.
 
  
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2996600 parameters</partinfo>
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<partinfo>BBa_K2996703 parameters</partinfo>
 
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Revision as of 06:43, 6 October 2019


RpoA, DNA-directed RNA polymerase subunit alpha

RpoA encodes the DNA-directed RNA polymerase subunit alpha, which plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme. It may activate transcription more effectively, compared to RpoZ used in other expereiments.

This RpoA-dCas9 fusion protein should be an inovated transcription activator.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 459
  • 1000
    COMPATIBLE WITH RFC[1000]