Difference between revisions of "Part:BBa K2992013"
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===References=== | ===References=== | ||
| − | Zuker, 2003 - | + | Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research, 31(13), pp.3406-3415. |
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'''Measurement''' | '''Measurement''' | ||
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured] | * [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured] | ||
Revision as of 13:22, 21 October 2019
Usage
Tfad is a terminator sequence derived from the fad gene of C. tetani, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the pyrE operon of C. sporogenes following chromosomal knock-in of botR (BBa_K2992002) at this locus. The secondary structure for Tfad was predicted using the Mfold web tool (Zuker, 2003).
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Tfad from C. tetani
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Secondary Structure
References
Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research, 31(13), pp.3406-3415.
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]