Difference between revisions of "Part:BBa K2992020"

 
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<partinfo>BBa_K2992020 short</partinfo>
  
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PbgaR-PbgaL regulatory system comprising divergent two divergent promoters with undefined 5’UTR and RBS regions. 
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===Usage and Biology===
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The BgaR-BgaL system of <i>C. perfringens</i>  comprises the transcriptional regulator BgaR belonging to the AraC-family and the β-galactosidase BgaL which are transcribed in a regulated fashion from the divergent P<i>gaR</i> -P<i>gaL</i>  promoter. The BgaR-BgaL system regulates the expression of carbohydrate metabolic genes in response to lactose concentrations (Hartman and Melville 2011). This parts entry represents the core regulatory component comprising the divergent P<i>gaR</i> -P<i>gaL</i>  promoter coupled with their non-defined 5’-UTR and RBS regions. Our group has recently utlised this regulatory system in order to generate a tightly regulate inducible system for CRISPR-Cas mutagenesis in the genus <i>Clostridium</i> (Cañadas et al., 2019). In our project, we use the P<i>gaR</i> -P<i>gaL</i>  regulatory unit in conjunction with its cognate transcriptional regulator <i>bgaR</i>  (hyperlinks and descriptions) to drive the expression of our volatile and FAST reporter genes in an inducible fashion. Doing so helps us fulfil our goal of generating reporter strains for the prediction of botulinum neurotoxin production following food manufacture.  <rb><br>
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===Characterisation===
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Data incoming
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2992020 SequenceAndFeatures</partinfo>
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===References===
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Cañadas et al., 2019 RiboCas - update
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Hartman and Melville 2011.
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===Functional Parameters===
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<partinfo>BBa_K2992020 parameters</partinfo>
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Revision as of 14:48, 16 September 2019

PbgaR component of PbgaR -PbgaL from C. perfringens

PbgaR-PbgaL regulatory system comprising divergent two divergent promoters with undefined 5’UTR and RBS regions.


Usage and Biology

The BgaR-BgaL system of C. perfringens comprises the transcriptional regulator BgaR belonging to the AraC-family and the β-galactosidase BgaL which are transcribed in a regulated fashion from the divergent PgaR -PgaL promoter. The BgaR-BgaL system regulates the expression of carbohydrate metabolic genes in response to lactose concentrations (Hartman and Melville 2011). This parts entry represents the core regulatory component comprising the divergent PgaR -PgaL promoter coupled with their non-defined 5’-UTR and RBS regions. Our group has recently utlised this regulatory system in order to generate a tightly regulate inducible system for CRISPR-Cas mutagenesis in the genus Clostridium (Cañadas et al., 2019). In our project, we use the PgaR -PgaL regulatory unit in conjunction with its cognate transcriptional regulator bgaR (hyperlinks and descriptions) to drive the expression of our volatile and FAST reporter genes in an inducible fashion. Doing so helps us fulfil our goal of generating reporter strains for the prediction of botulinum neurotoxin production following food manufacture.

Characterisation

Data incoming

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Cañadas et al., 2019 RiboCas - update Hartman and Melville 2011.