Difference between revisions of "Part:BBa K2992019"

 
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Transcriptional regulator <i>bgaR</i> associated with a lactose inducible system from <i>C. perfringens</i>   
 
Transcriptional regulator <i>bgaR</i> associated with a lactose inducible system from <i>C. perfringens</i>   
  
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===Usage and Biology===
 
===Usage and Biology===
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BgaR is the transcriptional regulator associated with the BgaR-BgaL lactose inducible system found naturally on the genome of <i>C. perfringens</i>. Our group has recently utlised this regulatory system in order to generate a tightly regulate inducible system for CRISPR-Cas mutagenesis in the genus <i>Clostridium</i> ((Cañadas et al., 2019). In our project, we use <i>bgaR</i> as part of the entire regulatory unit comprising <i>bgaR</i>, and its divergent promoter P<i>bgaR</i> -P<i>bgaL</i>  with their associated 5’-UTR and RBS regions (hyperlinks and descriptions) to drive the expression of our volatile and FAST reporter genes in an inducible fashion. Doing so helps us fulfil our goal of generating reporter strains for the prediction of botulinum neurotoxin production following food manufacture. <rb><br>
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===Characterisation===
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Data incoming
  
 
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<partinfo>BBa_K2992019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2992019 SequenceAndFeatures</partinfo>
  
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===References===
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Cañadas et al., 2019 RiboCas - update
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Hartman and Melville 2011.
  
 
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Revision as of 14:17, 16 September 2019


bgaR gene from C. perfringens

Transcriptional regulator bgaR associated with a lactose inducible system from C. perfringens


Usage and Biology

BgaR is the transcriptional regulator associated with the BgaR-BgaL lactose inducible system found naturally on the genome of C. perfringens. Our group has recently utlised this regulatory system in order to generate a tightly regulate inducible system for CRISPR-Cas mutagenesis in the genus Clostridium ((Cañadas et al., 2019). In our project, we use bgaR as part of the entire regulatory unit comprising bgaR, and its divergent promoter PbgaR -PbgaL with their associated 5’-UTR and RBS regions (hyperlinks and descriptions) to drive the expression of our volatile and FAST reporter genes in an inducible fashion. Doing so helps us fulfil our goal of generating reporter strains for the prediction of botulinum neurotoxin production following food manufacture.

Characterisation

Data incoming

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 368
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 368
    Illegal BglII site found at 428
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 368
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 368
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Cañadas et al., 2019 RiboCas - update Hartman and Melville 2011.