Difference between revisions of "Part:BBa K2992013"
m |
|||
| Line 17: | Line 17: | ||
[[Image:Mfold-K2992013-1.png]] | [[Image:Mfold-K2992013-1.png]] | ||
| + | ===References=== | ||
| + | Zuker, 2003 - update | ||
<hr> | <hr> | ||
'''Measurement''' | '''Measurement''' | ||
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured] | * [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured] | ||
Revision as of 16:09, 16 September 2019
Usage
Tfad is a terminator sequence derived from the fad gene of C. tetani, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the pyrE operon of C. sporogenes following chromosomal knock-in of botR (BBa_K2992002) at this locus. The secondary structure for Tfad was predicted using the Mfold web tool (Zuker, 2003).
|
|
Tfad from C. tetani
|
Secondary Structure
References
Zuker, 2003 - update
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]