Difference between revisions of "Part:BBa K3128004:Design"
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PLac-RBS and the termintors are from iGEM plates from Bba_J04450. | PLac-RBS and the termintors are from iGEM plates from Bba_J04450. | ||
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NanoLuc gene is from Promega vector's pNL1.1[Nluc] | NanoLuc gene is from Promega vector's pNL1.1[Nluc] | ||
===References=== | ===References=== | ||
| + | https://www.ncbi.nlm.nih.gov/nuccore/JQ437370 | ||
Revision as of 09:53, 19 September 2019
NanoLuciferase reporter for BACTH assay (with two restriction enzymes around the reporter)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 229
Illegal BamHI site found at 769 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
BBA J04450 was used for its CAP dependant Lactose Promoter (PLac) and it's strong RBS making it able to produce a large amount of protein when activated by IPTG and cAMP-CAP complexes. Two restrictions sites were added on the biobrick to to remove the RFP gene and replace it by the NanoLuciferase gene, BglII in 3' and BamHI in 5'.
Those sites are still in the biobrick.
Source
PLac-RBS and the termintors are from iGEM plates from Bba_J04450.
NanoLuc gene is from Promega vector's pNL1.1[Nluc]