Difference between revisions of "Part:BBa I746916:Experience"
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[[Image:T--Linkoping_Sweden--CBD-sfGFPstorodl4.jpeg|300px|thumb|left|<b><i>Figure 1.</i></b> 1 liter growth medium containing induced CBD-sfGFP BL21 (DE3) on a UV-table. ]] | [[Image:T--Linkoping_Sweden--CBD-sfGFPstorodl4.jpeg|300px|thumb|left|<b><i>Figure 1.</i></b> 1 liter growth medium containing induced CBD-sfGFP BL21 (DE3) on a UV-table. ]] | ||
| − | + | <b>Figure 1</b> depict a 1 liter culture of induced CBD-sfGFP E.coli BL21(DE3) on a UV-table. The picture shows that an induced culture of CBD-sfGFP emits fluorescence at 302 nm. | |
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Revision as of 09:35, 6 October 2019
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_I746916
2019 iGEM team Linkoping Sweden
Figure 1 depict a 1 liter culture of induced CBD-sfGFP E.coli BL21(DE3) on a UV-table. The picture shows that an induced culture of CBD-sfGFP emits fluorescence at 302 nm.
Pictures above shows BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP at different IPTG concentrations between 0.05 - 1.0 mM. The induction gradient for Vibrio is more correlated with the IPTG concentration then E.coli suggesting that the induction windows for expressing CBD-sfGFP are different between E.coli BL21 (DE3) and Vibrio natriegens Vmax.
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