Difference between revisions of "Part:BBa I746916:Experience"

(→‎Applications of BBa_I746916)
(e)
Line 8: Line 8:
 
<h2> 2019 iGEM team Linkoping Sweden </h2>
 
<h2> 2019 iGEM team Linkoping Sweden </h2>
  
−
[[Image:T--Linkoping_Sweden--CBD-sfGFPstorodl4.jpeg|300px|thumb|left|<b><i>Figure 1.</i></b> 1 liter growth medium containing CBD-sfGFP BL21 (DE3) (induced?) on a UV-table. ]]  
+
[[Image:T--Linkoping_Sweden--CBD-sfGFPstorodl4.jpeg|300px|thumb|left|<b><i>Figure 1.</i></b> 1 liter growth medium containing induced CBD-sfGFP BL21 (DE3) on a UV-table. ]]  
−
<br> Pictures above shows induced BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP. The picture to the left is a 1 liter growth medium with E.coli BL21 expressing CBD-sfGFP. The picture in the middle is a depiction of an induction gradient with BL21 with varying IPTG concentrations between 0.05-1.0 mM. The picture to the left is a 1 liter culture of induced Vibtio natriegens
+
  
−
The chosen IPTG concentrations were XX mM for e.coli and Vibrio natriegens
+
The picture to the left is a 1 liter culture of induced CBD-sfGFP e.coli BL21(DE3).
  
−
The induction gradient for Vibrio is more correlated with the IPTG concentration then E.Coli suggesting that the induction windows are different between the bacteria with CBD-sfGFP.  
+
<br> Pictures above shows BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP at different IPTG concentrations between 0.05 - 1.0 mM. The induction gradient for Vibrio is more correlated with the IPTG concentration then E.coli suggesting that the induction windows for expressing CBD-sfGFP are different between E.coli BL21 (DE3) and Vibrio natriegens Vmax.
 +
 
 +
  
  

Revision as of 15:33, 13 September 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I746916

2019 iGEM team Linkoping Sweden

Figure 1. 1 liter growth medium containing induced CBD-sfGFP BL21 (DE3) on a UV-table.

The picture to the left is a 1 liter culture of induced CBD-sfGFP e.coli BL21(DE3).


Pictures above shows BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP at different IPTG concentrations between 0.05 - 1.0 mM. The induction gradient for Vibrio is more correlated with the IPTG concentration then E.coli suggesting that the induction windows for expressing CBD-sfGFP are different between E.coli BL21 (DE3) and Vibrio natriegens Vmax.



































User Reviews

UNIQ28a6109ac05f3733-partinfo-00000000-QINU UNIQ28a6109ac05f3733-partinfo-00000001-QINU