Difference between revisions of "Part:BBa K3030012"
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<partinfo>BBa_K3030012 short</partinfo> | <partinfo>BBa_K3030012 short</partinfo> | ||
| − | This part is a combination of MS2 ribozyme switch and PP7 coat protein to manipulate regulation process with another part automatically in the | + | This part is a combination of MS2 ribozyme switch and PP7 coat protein to manipulate regulation process with another part automatically in the mammalian cells. We built up a three plasmids circuit which aimed to subsequently regulate expression level of gene(s) connected by 2A peptide with regulating protein (MS2/PP7). |
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Revision as of 12:27, 14 October 2019
MS2-responsive ribozyme switch (Off to On) + PP7 coat protein
This part is a combination of MS2 ribozyme switch and PP7 coat protein to manipulate regulation process with another part automatically in the mammalian cells. We built up a three plasmids circuit which aimed to subsequently regulate expression level of gene(s) connected by 2A peptide with regulating protein (MS2/PP7).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]