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5’-UTR containing RBS for <i>ntnH</i> gene from <i>C. botulinum</i>   
 
5’-UTR containing RBS for <i>ntnH</i> gene from <i>C. botulinum</i>   

Revision as of 17:19, 12 September 2019


5-UTR containing RBS for ntnH gene from C. botulinum

5’-UTR containing RBS for ntnH gene from C. botulinum


Usage and Biology

The native 5’-UTR containing the RBS is found naturally upstream of ntnH in C. botulinum. ntnH encodes the non-toxic non-haemagglutinin component of the botulinum neurotoxin complexes. Its expression is directly regulated by BotR. In our project we use the regulatory region of ntnH to drive expression of the toxin regulator botR and our chosen reporter genes of interest which have been placed under the control of the neurotoxin and neurotoxin-associated promoters. Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.

Characterisation

Data incoming.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Raffestin et al., 2005 (update)