Difference between revisions of "Part:BBa K2992001"
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Revision as of 16:53, 12 September 2019
PntnH
Promoter region for the non-toxic nonhemmagluttinin component of the neurotoxin gene cluster in Clostridum botulinum.
Usage and Biology
Pntnh is found upstream of the 5'-UTR and RBS of the nonhemmagluttinin component (ntnh) of the neurotoxin gene cluster in C. botulinum. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest which have been placed under the control of the neurotoxin and neurotoxin-associated promoters. Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.
Characterisation
Data incoming.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Raffestin et al., 2005 (update)