Difference between revisions of "Part:BBa K2992001"

Revision as of 16:47, 12 September 2019

PntnH

Promoter region for the non-toxic nonhemmagluttinin component of the neurotoxin gene cluster in Clostridum botulinum.

Usage and Biology

Pntnh is found upstream of the 5'-UTR and RBS of the nonhemmagluttinin component (ntnh) of the neurotoxin gene cluster in C. botulinum. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest which have been placed under the control of the neurotoxin and neurotoxin-associated promoters. Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.

Characterisation

Data incoming.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-P<i>ntnh</i> is found upstream of the 5'-UTR and RBS of the thenonhemmagluttinin component (ntnh) of the neurotoxin gene cluster in <i>C. botulinum</i>. This promoter was used to drive expression of of the toxin regulator <i>botR</i> and our chosen reporter genes of interest which have been placed under the control of the neurotoxin and neurotoxin-associated promoters. Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.
+P<i>ntnh</i> is found upstream of the 5'-UTR and RBS of the nonhemmagluttinin component (ntnh) of the neurotoxin gene cluster in <i>C. botulinum</i>. This promoter was used to drive expression of of the toxin regulator <i>botR</i> and our chosen reporter genes of interest which have been placed under the control of the neurotoxin and neurotoxin-associated promoters. Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.
 ===Characterisation===