Difference between revisions of "Part:BBa K2923002"

 
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<partinfo>BBa_K2923002 short</partinfo>
 
<partinfo>BBa_K2923002 short</partinfo>
  
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an aptazyme is an aptamer-ribozyme combinations with intrinsic RNA cleavage activity. this aptazyme is specific for the theophylline (liguant). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine He is composed in 3 differents part, a twister ribozyme module, a communication module and an aptamer module This aptazyme is an inactive aptazyme, this mean that the ribozyme part is mutated and the aptazyme lost his capacity to self-cleave. you can use it as a negative control fot the part: BBa_K2923001
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An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. this aptazyme is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. It is composed in 3 different part, a twister ribozyme module, a communication module, and an aptamer module This sequence correspond to inactive aptazyme, this means that the ribozyme part is mutated and the aptazyme lost his capacity to self-cleave. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923001
  
  

Revision as of 21:53, 12 October 2019


Theophylline inactif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. this aptazyme is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. It is composed in 3 different part, a twister ribozyme module, a communication module, and an aptamer module This sequence correspond to inactive aptazyme, this means that the ribozyme part is mutated and the aptazyme lost his capacity to self-cleave. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923001


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]