Difference between revisions of "Part:BBa K2963018:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Bacillus Genome.
+
Derived from Bacillus subtilis.
  
 
===References===
 
===References===

Latest revision as of 07:00, 12 October 2019


pgsB - encoding a subunit of poly-γ-glutamic acid synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1032
    Illegal PstI site found at 1013
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1013
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1032
    Illegal PstI site found at 1013
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1032
    Illegal PstI site found at 1013
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we express the PgsC protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.


Source

Derived from Bacillus subtilis.

References