Difference between revisions of "Part:BBa K2963018"

Revision as of 11:53, 10 October 2019

pgsB - encoding a subunit of poly-γ-glutamic acid synthetase

This part contains the pgsB gene for poly-gamma-glutamic acid synthetase. This enzyme is involved in poly-gamma-glutamic acid synthesis,which converts the glutamate to poly-gamma-glutamic acid. PgsB is mainly responsible for catalysing poly-gamma-glutamic acid synthesis. In our subject, we get the pgsB sequenece by error-prone PCR of Bacillus genome, trying to improve the activity of PgsB protein.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1032
Illegal PstI site found at 1013
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1013
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1032
Illegal PstI site found at 1013
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1032
Illegal PstI site found at 1013
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2963018 short</partinfo>
-This part contains the pgsC gene for poly-gamma-glutamic acid synthetase from Bacillus licheniformis. This enzyme is involved in poly-gamma-glutamic synthesis,which converts the glutamate to poly-gamma-glutamic acid.As a hydrophobic protein,PgsC is located in the cytomembrane and hypothesized to connect the PgsB and PgsA to form the PgsBCA complex.
+This part contains the pgsB gene for poly-gamma-glutamic acid synthetase. This enzyme is involved in poly-gamma-glutamic acid synthesis,which converts the glutamate to poly-gamma-glutamic acid. PgsB is mainly responsible for catalysing poly-gamma-glutamic acid synthesis. In our subject, we get the pgsB sequenece by error-prone PCR of Bacillus genome, trying to improve the activity of PgsB protein.
 <!-- Add more about the biology of this part here