Difference between revisions of "Part:BBa K2522001:Experience"

Revision as of 02:26, 11 September 2019

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2522001

While this part was not applied to our project, it did give our team insight in to our future work with the sfGFP gene. This plasmid was experimentally tested and proved to work by growing the colony up overnight with 3OC12, and a control without. The visual results are below.

2019 US AFRL CarrollHS

We created six overnights that contained 3 mL of LB Broth, 3 μL of the antibiotic Chloramphenicol, E. coli DH5α cells transformed with PLas_sfGFP, and either 3 μL of 10 mM 3OC12 (three overnights) or 3 μL of DMSO (three overnights) to confirm the functionality of the plasmid and one 3 mL overnight that contained only LB Broth to serve as our blank.

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Through these overnights, we were able to confirm the functionality of PLas_sfGFP and we measured the fluorescence and absorbance of each overnight by performing an endpoint read of each overnight in a 96-well flat-bottomed plate. In order to obtain data in the range of our standard curve, we diluted the overnights two-fold by mixing 50 μL of each overnight with 50 μL of LB.

User Reviews

UNIQ6386ae38a769deec-partinfo-00000000-QINU UNIQ6386ae38a769deec-partinfo-00000001-QINU

@@ Line 12: / Line 12: @@
 [[File:3OC12overnightsNBL.jpeg|400px|left|<p align="justify">'</p>]]
 [[File:3OC12overnightsBL.jpeg|400px|center|<p align="justify">'</p>]]‎
+Through these overnights, we were able to confirm the functionality of PLas_sfGFP and we measured the fluorescence and absorbance of each overnight by performing an endpoint read of each overnight in a 96-well flat-bottomed plate. In order to obtain data in the range of our standard curve, we diluted the overnights two-fold by mixing 50 μL of each overnight with 50 μL of LB.
 ===User Reviews===