Difference between revisions of "Part:BBa K2522001:Experience"
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===2019 US AFRL CarrollHS=== | ===2019 US AFRL CarrollHS=== | ||
We created six overnights that contained 3 mL of LB Broth, 3 μL of the antibiotic Chloramphenicol, E. coli DH5α cells transformed with PLas_sfGFP, and either 3 μL of 10 mM 3OC12 (three overnights) or 3 μL of DMSO (three overnights) to confirm the functionality of the plasmid and one 3 mL overnight that contained only LB Broth to serve as our blank. | We created six overnights that contained 3 mL of LB Broth, 3 μL of the antibiotic Chloramphenicol, E. coli DH5α cells transformed with PLas_sfGFP, and either 3 μL of 10 mM 3OC12 (three overnights) or 3 μL of DMSO (three overnights) to confirm the functionality of the plasmid and one 3 mL overnight that contained only LB Broth to serve as our blank. | ||
| + | [[File:3OC12overnightsNBL.jpeg|400px|left|<p align="justify">'</p>]] | ||
| + | [[File:3OC12overnightsBL.jpeg|400px|center|<p align="justify">'</p>]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 02:24, 11 September 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2522001
While this part was not applied to our project, it did give our team insight in to our future work with the sfGFP gene. This plasmid was experimentally tested and proved to work by growing the colony up overnight with 3OC12, and a control without. The visual results are below.
2019 US AFRL CarrollHS
We created six overnights that contained 3 mL of LB Broth, 3 μL of the antibiotic Chloramphenicol, E. coli DH5α cells transformed with PLas_sfGFP, and either 3 μL of 10 mM 3OC12 (three overnights) or 3 μL of DMSO (three overnights) to confirm the functionality of the plasmid and one 3 mL overnight that contained only LB Broth to serve as our blank.
User Reviews
UNIQaf907abefbb79045-partinfo-00000000-QINU UNIQaf907abefbb79045-partinfo-00000001-QINU