Difference between revisions of "Part:BBa K3145002:Experience"
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===Applications of BBa_K3145002=== | ===Applications of BBa_K3145002=== | ||
After we confirmed through sequencing that we removed the XbaI cut site between the T7 promoter and RBS region, we created six overnights that contained 3 mL of LB Broth, 3 µL of the antibiotic Kanamycin (50 ng/µL) and either E.coli DH5α cells containing T7mut-sfGFP (three overnights) or either E.coli DH5α cells containing pY71-sfGFP(three overnights) to compare the functionality between the two plasmids, and one overnight that only contained 3 mL of LB Broth only to serve as our blank. | After we confirmed through sequencing that we removed the XbaI cut site between the T7 promoter and RBS region, we created six overnights that contained 3 mL of LB Broth, 3 µL of the antibiotic Kanamycin (50 ng/µL) and either E.coli DH5α cells containing T7mut-sfGFP (three overnights) or either E.coli DH5α cells containing pY71-sfGFP(three overnights) to compare the functionality between the two plasmids, and one overnight that only contained 3 mL of LB Broth only to serve as our blank. | ||
| − | [[File:T7mutovernights.jpeg| | + | [[File:T7mutovernights.jpeg|650px|center|thumb|''' |
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K3145002 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K3145002 StartReviews</partinfo> | ||
Revision as of 01:28, 11 September 2019
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K3145002
After we confirmed through sequencing that we removed the XbaI cut site between the T7 promoter and RBS region, we created six overnights that contained 3 mL of LB Broth, 3 µL of the antibiotic Kanamycin (50 ng/µL) and either E.coli DH5α cells containing T7mut-sfGFP (three overnights) or either E.coli DH5α cells containing pY71-sfGFP(three overnights) to compare the functionality between the two plasmids, and one overnight that only contained 3 mL of LB Broth only to serve as our blank. [[File:T7mutovernights.jpeg|650px|center|thumb|
User Reviews
UNIQ3723bcefe8e699a5-partinfo-00000000-QINU UNIQ3723bcefe8e699a5-partinfo-00000001-QINU