Difference between revisions of "Part:BBa K3056001:Design"
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===References=== | ===References=== | ||
+ | 1. Brennan, J. (2005) The production of recombinant single chain antibody fragments for the detection of illicit drug residues. doctoral thesis, Dublin City University, [online] http://doras.dcu.ie/17319/ (Accessed March 12, 2019) |
Latest revision as of 20:12, 11 October 2019
Anti-THC antibody fragment (ScFv), bound to EGFP at the C-terminal
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 691
Illegal AgeI site found at 1380 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 820
Design Notes
EGFP was chosen over other fluorescent protein, as it is brighter than most.
Source
The antibody fragment was obtained from "The Production of Recombinant single chain Antibody Fragments for the Detection of Illicit Drug Residues", written by Dr. Brennan.
References
1. Brennan, J. (2005) The production of recombinant single chain antibody fragments for the detection of illicit drug residues. doctoral thesis, Dublin City University, [online] http://doras.dcu.ie/17319/ (Accessed March 12, 2019)