Difference between revisions of "Part:BBa K2992013"

 
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===Usage===
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T<i>fad</i>  is a terminator sequence derived from the <i>fad</i> gene of <i>C. tetani</i>, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the <i>pyrE</i>  operon of <i>C. sporogenes</i>  following chromosomal knock-in of <i>botR</i>  (insert hyperlink) at this locus.
 
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Revision as of 16:17, 10 September 2019

Usage

Tfad is a terminator sequence derived from the fad gene of C. tetani, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the pyrE operon of C. sporogenes following chromosomal knock-in of botR (insert hyperlink) at this locus.

Terminator.png

Tfad from C. tetani

  • Transcriptional terminator sequence from the FAD-dependent oxidoreductase, fad, from Clostridium tetani

Secondary Structure

Mfold-K2992013-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]