Difference between revisions of "Part:BBa K3111002"

 
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<partinfo>BBa_K3111002 short</partinfo>
 
<partinfo>BBa_K3111002 short</partinfo>
  
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StrepII tag is used to for protein purification while enterokinase site allows for post-purification removal of the tag
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Modification to Team EPFL 2018 part BBa_K2686001 with addition of a StrepII tag to purify assembled encapsulin without heat treatment. Enterokinase site allows for post-purification removal of the tag.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:58, 10 September 2019


T. maritima encapsulin from BBa_K2686001 (no stop codon)

Modification to Team EPFL 2018 part BBa_K2686001 with addition of a StrepII tag to purify assembled encapsulin without heat treatment. Enterokinase site allows for post-purification removal of the tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 375
    Illegal SapI.rc site found at 406