Difference between revisions of "Part:BBa K2967005:Experience"
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Amplifier and GFP are cloned on pcdfDuet-1 vectors which are called V-AM and V-GFP. Then empty plasmid(VE), V-GFP and V-AM are transformed into E. coli BL-21, and single colonies are added to 5 ml LB medium for overnight incubation at 37 degrees Celsius. Using 1 ml PBS to wash the bacteria and measuring the vale of OD600 to obtain cell concentration of each culture medium. The bacterial culture medium was diluted to OD600=0.025 with LBS(LB with streptomycin) and then added to black 96-well plate. IPTG with concentration of 0, 1*10-3, 1*10-4, 1*10-5 and 1*10-6 was added to induce those bacteria. After incubation for 4 hours and 6hours, the data were detected by enzyme labeling instrument. The excitation wavelength is 485 nm and the absorption wavelength is 525 nm. | Amplifier and GFP are cloned on pcdfDuet-1 vectors which are called V-AM and V-GFP. Then empty plasmid(VE), V-GFP and V-AM are transformed into E. coli BL-21, and single colonies are added to 5 ml LB medium for overnight incubation at 37 degrees Celsius. Using 1 ml PBS to wash the bacteria and measuring the vale of OD600 to obtain cell concentration of each culture medium. The bacterial culture medium was diluted to OD600=0.025 with LBS(LB with streptomycin) and then added to black 96-well plate. IPTG with concentration of 0, 1*10-3, 1*10-4, 1*10-5 and 1*10-6 was added to induce those bacteria. After incubation for 4 hours and 6hours, the data were detected by enzyme labeling instrument. The excitation wavelength is 485 nm and the absorption wavelength is 525 nm. | ||
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===Applications of BBa_K2967005=== | ===Applications of BBa_K2967005=== | ||
Revision as of 08:40, 10 September 2019
Amplifier and GFP are cloned on pcdfDuet-1 vectors which are called V-AM and V-GFP. Then empty plasmid(VE), V-GFP and V-AM are transformed into E. coli BL-21, and single colonies are added to 5 ml LB medium for overnight incubation at 37 degrees Celsius. Using 1 ml PBS to wash the bacteria and measuring the vale of OD600 to obtain cell concentration of each culture medium. The bacterial culture medium was diluted to OD600=0.025 with LBS(LB with streptomycin) and then added to black 96-well plate. IPTG with concentration of 0, 1*10-3, 1*10-4, 1*10-5 and 1*10-6 was added to induce those bacteria. After incubation for 4 hours and 6hours, the data were detected by enzyme labeling instrument. The excitation wavelength is 485 nm and the absorption wavelength is 525 nm.
Applications of BBa_K2967005
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