Difference between revisions of "Part:BBa K3020000:Experience"
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Typical DNA damage agents include nalidixic acid (NA) (Sigma), formaldehyde (formaldehyde, CH2O) (Sigma) and hydrogen peroxide(H2O2).The following non-DNA damage reagent was used as a negative control:kanamycin sulfate，KAN）（Amresco）,acetone,phenol. | Typical DNA damage agents include nalidixic acid (NA) (Sigma), formaldehyde (formaldehyde, CH2O) (Sigma) and hydrogen peroxide(H2O2).The following non-DNA damage reagent was used as a negative control:kanamycin sulfate，KAN）（Amresco）,acetone,phenol. | ||
The reagent concentration and gradient were calculated and added to the 40 ml bacterial solution (LB) system according to the following induction volume. | The reagent concentration and gradient were calculated and added to the 40 ml bacterial solution (LB) system according to the following induction volume. | ||
| − | [[File:BIT201906-youdaoji1.jpeg| | + | [[File:BIT201906-youdaoji1.jpeg|thumb|400px|center|'''DNA damage agent concentration gradient and induced addition''']] |
| − | [[File:BIT201907-youdao2.jpeg| | + | [[File:BIT201907-youdao2.jpeg|thumb|400px|center|'''non-DNA damage agent concentration gradient and induced addition''']] |
Revision as of 08:10, 10 September 2019
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K3020000
User Reviews
Typical DNA damage agents include nalidixic acid (NA) (Sigma), formaldehyde (formaldehyde, CH2O) (Sigma) and hydrogen peroxide(H2O2).The following non-DNA damage reagent was used as a negative control:kanamycin sulfate，KAN）（Amresco）,acetone,phenol. The reagent concentration and gradient were calculated and added to the 40 ml bacterial solution (LB) system according to the following induction volume.
