Difference between revisions of "Part:BBa K2938000:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
None, this promoter was already in use in our lab.
+
None
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Schlesinger, Orr, et al. "Tuning of recombinant protein expression in Escherichia coli by manipulating transcription, translation initiation rates, and incorporation of noncanonical amino acids." ACS synthetic biology 6.6 (2017): 1076-1085.
+
This promoter was already in use in our lab.
−
https://www.biorxiv.org/content/10.1101/100982v1.full
+
  
  
 
===References===
 
===References===
 +
Schlesinger, Orr, et al. "Tuning of recombinant protein expression in Escherichia coli by manipulating transcription, translation initiation rates, and incorporation of noncanonical amino acids." ACS synthetic biology 6.6 (2017): 1076-1085.
 +
https://www.biorxiv.org/content/10.1101/100982v1.full

Revision as of 08:55, 28 September 2019


Attenuated pBEST-PR promoter after point mutation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

This promoter was already in use in our lab.


References

Schlesinger, Orr, et al. "Tuning of recombinant protein expression in Escherichia coli by manipulating transcription, translation initiation rates, and incorporation of noncanonical amino acids." ACS synthetic biology 6.6 (2017): 1076-1085. https://www.biorxiv.org/content/10.1101/100982v1.full