Difference between revisions of "Part:BBa K3257000"
| Line 5: | Line 5: | ||
Promoter for bacteriophage T7 RNA polymerase. | Promoter for bacteriophage T7 RNA polymerase. | ||
| + | '''Biology''' | ||
| + | [[File:Fluorescence of EGFP.jpeg|center|400px|thumb|'''Figure 2. The relative fluorescence intensity of EGFP measured by 96-well plate reader''' EGFP represents the relatively fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relatively fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 20:25, 21 October 2019
T7 Promoter
Promoter for bacteriophage T7 RNA polymerase.
Biology
Figure 2. The relative fluorescence intensity of EGFP measured by 96-well plate reader EGFP represents the relatively fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relatively fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]