Difference between revisions of "Part:BBa K2963009:Design"

 
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===Design Notes===
 
===Design Notes===
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In order to express PgsBCA complex heterologously in corynebacterium glutamicum, we add Ptac promoter with a short RBS before the start codon and T7 terminater of each protein， respectively.
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In order to express CapBCA complex heterologously in corynebacterium glutamicum, we add Ptac promoter with a short RBS before the start codon and T7 terminater of each protein， respectively.
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===Source===
 
===Source===

Revision as of 06:53, 12 October 2019


capB*CA - encoding poly-γ-glutamic acid synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
    Illegal NheI site found at 2365
    Illegal NheI site found at 2963
    Illegal NheI site found at 4281
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
    Illegal NgoMIV site found at 2590
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to express CapBCA complex heterologously in corynebacterium glutamicum, we add Ptac promoter with a short RBS before the start codon and T7 terminater of each protein， respectively.

Source

Bacillus Genome.

References