Difference between revisions of "Part:BBa K2933102"

 
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<partinfo>BBa_K2933102 short</partinfo>
 
<partinfo>BBa_K2933102 short</partinfo>
  
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This part encodes the fusion protein of GST tag and SPG-1 to promote the expression and purification of target protein(SPG-1).  
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This part encodes the fusion protein of His-Sumo tag and SPG-1 to promote the expression and purification of target protein(SPG-1).  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:23, 6 September 2019


His+Linker a+Sumo+Linker b+SPG-1

This part encodes the fusion protein of His-Sumo tag and SPG-1 to promote the expression and purification of target protein(SPG-1).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 256
    Illegal NheI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 256
    Illegal BglII site found at 145
    Illegal BamHI site found at 344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]