Difference between revisions of "Part:BBa K2933011"
| Line 8: | Line 8: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The German imipenemase-1 (GIM-1) MBL was first identified in clinical isolates of Pseudomonas aeruginosa in Germany in 2002 | |
| + | Recently, GIM-1 has been identified in other bacterial species, such as Serratia marcescens, Enterobacter cloacae, and Acinetobacter pittii , indicating that it is transmitted on mobile genetic elements. | ||
| + | GIM-2 is a new variant of GIM-1 with a single mutation, A290G, which was recently discovered. | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 06:38, 6 September 2019
subclass B1 metallo-beta-lactamase GIM-2, codon optimized in E. coli
This part encodes a protein called GIM-2, which is a metallo-beta-lactamase of subclass B1.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 731
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 731
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 731
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 731
- 1000COMPATIBLE WITH RFC[1000]