Difference between revisions of "Part:BBa K2992001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | P<i>ntnh</i> is derived from the nerotoxin gene cluster of <i>C. botulinum</i>. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest in order to generate a volatile reporter strain to predict neurotoxin production following food processing. | + | P<i>ntnh</i> is derived from the nerotoxin gene cluster of <i>C. botulinum</i>. This promoter was used to drive expression of of the toxin regulator <i>botR</i> and our chosen reporter genes of interest in order to generate a volatile reporter strain to predict neurotoxin production following food processing. |
===Characterisation=== | ===Characterisation=== |
Revision as of 12:29, 9 September 2019
PntnH
Regulatory region comprising promoter 5'-UTR, and RBS for the non-toxic nonhemmagluttinin component of the neurotoxin gene cluster in Clostridum botulinum.
Usage and Biology
Pntnh is derived from the nerotoxin gene cluster of C. botulinum. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest in order to generate a volatile reporter strain to predict neurotoxin production following food processing.
Characterisation
Data incoming.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]