Difference between revisions of "Part:BBa K2992000"
Line 7: Line 7:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from <i>Halorhodospira halophila</i> and has been codon optimised for fluorescence studies in the genus <i>Clostridium</i>. In our project we use FAST as a reporter to demosntrate the activity of our chosen promoters and to assess its suitability as an alternative non-volatile reporter system for predicting neurotoxin production.   
+
FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from <i>Halorhodospira halophila</i> and has been codon optimised for fluorescence studies in the genus <i>Clostridium</i>. In our project we use FAST as a reporter to demosntrate the activity of our chosen promoters and to assess its suitability as an alternative non-volatile reporter system for predicting neurotoxin production.  <br><br><br>
 +
 
 +
===Characterisation===
 +
Date incoming <br><br><br>
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K2992000 parameters</partinfo>
 +
<!-- -->
 +
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2992000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2992000 SequenceAndFeatures</partinfo>

Revision as of 12:30, 5 September 2019


FAST reporter gene

Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST) reporter gene

Usage and Biology

FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from Halorhodospira halophila and has been codon optimised for fluorescence studies in the genus Clostridium. In our project we use FAST as a reporter to demosntrate the activity of our chosen promoters and to assess its suitability as an alternative non-volatile reporter system for predicting neurotoxin production.


Characterisation

Date incoming


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 155


Characterisation

Date incoming


References

Street et al., 2019 to be updated