Difference between revisions of "Part:BBa K2992000"

Revision as of 12:11, 5 September 2019

FAST reporter gene

Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST) reporter gene

Usage and Biology

FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from Halorhodospira halophila and has been codon optimised for fluorescence studies in the genus Clostridium. In our project we use FAST as a reporter to demosntrate the activity of our chosen promoters and to assess its suitability as an alternative non-volatile reporter system for predicting neurotoxin production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 155

References

Street et al., 2019 to be updated

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 ===Usage and Biology===
-FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from <i>Halorhodospira halophila</i> and has been codon optimised for fluorescence studies in the genus <i>Clostridium</i>. In our project we use FAST as a reporter to demosntrate the activity of our chosen promoters and to assess its suitability as an alternative non-volatile reporter system for predicting neurotoxin production.
+FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from <i>Halorhodospira halophila</i> and has been codon optimised for fluorescence studies in the genus <i>Clostridium</i>. In our project we use FAST as a reporter to demosntrate the activity of our chosen promoters and to assess its suitability as an alternative non-volatile reporter system for predicting neurotoxin production.  <br> <br> <br>
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 <span class='h3bb'>Sequence and Features</span>