Difference between revisions of "Part:BBa K2976010"

Revision as of 05:00, 5 September 2019

Improved T2A

Usage and Biology

Oligopeptide “2A‐like” sequences are found in a range of RNA virus genomes controlling protein biogenesis and T2A derives from Thosea asigna virus. The 2A peptide consensus motif is extremely rare and is always associated with cleavage activity between the glycine and the proline. The insertion of T2A within a single open reading frame (ORF) produces multiple proteins. Besides, inserting a GSG linker before the T2A sequence significantly improves its self-cleaving efficiency and ensures complete cleavage. In 2019 CPU_CHINA project, we utilize P2A and T2A to co-express TLR1:TLR2:CD14 cluster on our designer cell membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-Oligopeptide “2A‐like” sequences are found in a range of RNA virus genomes controlling protein biogenesis and T2A derives from Thosea asigna virus. The 2A peptide consensus motif is extremely rare and is always associated with cleavage activity between the glycine and the proline. The insertion of T2A within a single open reading frame (ORF) produces multiple proteins. Besides, inserting a GSG linker before the T2A sequence significantly improves its self-cleaving efficiency and ensures complete cleavage. In 2019 CPU_CHINA project, we utilize P2A and T2A to co-express TLR1:TLR2:CD14 cluster on our designer cell membrane.
+<p>Oligopeptide “2A‐like” sequences are found in a range of RNA virus genomes controlling protein biogenesis and T2A derives from Thosea asigna virus. The 2A peptide consensus motif is extremely rare and is always associated with cleavage activity between the glycine and the proline. The insertion of T2A within a single open reading frame (ORF) produces multiple proteins. Besides, inserting a GSG linker before the T2A sequence significantly improves its self-cleaving efficiency and ensures complete cleavage. In 2019 CPU_CHINA project, we utilize P2A and T2A to co-express TLR1:TLR2:CD14 cluster on our designer cell membrane.
 </p>