Difference between revisions of "Part:BBa I746916:Experience"

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[[Image:T--Linkoping_Sweden--CBD-sfGFPstorodl4.jpeg|300px|thumb|left|<b><i>Figure 1.</i></b> 1 liter growth medium containing CBD-sfGFP BL21 (DE3) (induced?) on a UV-table. ]]  
 
[[Image:T--Linkoping_Sweden--CBD-sfGFPstorodl4.jpeg|300px|thumb|left|<b><i>Figure 1.</i></b> 1 liter growth medium containing CBD-sfGFP BL21 (DE3) (induced?) on a UV-table. ]]  
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<br> Pictures above shows induced BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP. The picture to the left is a 1 liter growth medium with E.coli BL21 expressing CBD-sfGFP. The picture in the middle is a depiction of an induction gradient with BL21 and varying IPTG concentrations. The picture to the right is the same but with Vibrio natriegens Vmax instead. The induction gradient for Vibrio is more correlated with the IPTG concentration then E.Coli suggesting that the induction windows are different between the bacteria with CBD-sfGFP.  
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<br> Pictures above shows induced BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP. The picture to the left is a 1 liter growth medium with E.coli BL21 expressing CBD-sfGFP. The picture in the middle is a depiction of an induction gradient with BL21 with varying IPTG concentrations between 0.05-1.0 mM. The picture to the left is a 1 liter culture of induced Vibtio natriegens  
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The chosen IPTG concentrations were XX mM for e.coli and Vibrio natriegens
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The induction gradient for Vibrio is more correlated with the IPTG concentration then E.Coli suggesting that the induction windows are different between the bacteria with CBD-sfGFP.  
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Revision as of 15:25, 13 September 2019


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Please enter how you used this part and how it worked out.

Applications of BBa_I746916

2019 iGEM team Linkoping Sweden

Figure 1. 1 liter growth medium containing CBD-sfGFP BL21 (DE3) (induced?) on a UV-table.


Pictures above shows induced BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP. The picture to the left is a 1 liter growth medium with E.coli BL21 expressing CBD-sfGFP. The picture in the middle is a depiction of an induction gradient with BL21 with varying IPTG concentrations between 0.05-1.0 mM. The picture to the left is a 1 liter culture of induced Vibtio natriegens

The chosen IPTG concentrations were XX mM for e.coli and Vibrio natriegens 

The induction gradient for Vibrio is more correlated with the IPTG concentration then E.Coli suggesting that the induction windows are different between the bacteria with CBD-sfGFP.


































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