Difference between revisions of "Part:BBa I746916:Experience"

Revision as of 13:56, 4 September 2019

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Applications of BBa_I746916

2019 iGEM team Linkoping Sweden

Figure 1. 1 liter growth medium containing CBD-sfGFP BL21 (DE3) (induced?) on a UV-table.

Pictures above shows induced BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP. The picture to the left is a 1 liter growth medium with E.coli BL21 expressing CBD-sfGFP. The picture in the middle is a depiction of an induction gradient with BL21 and varying IPTG concentrations. The picture to the right is the same but with Vibrio natriegens Vmax instead. The induction gradient for Vibrio is more correlated with the IPTG concentration then E.Coli suggesting that the induction windows are different between the bacteria with CBD-sfGFP.

User Reviews

UNIQ446c5e032e3a17a3-partinfo-00000000-QINU UNIQ446c5e032e3a17a3-partinfo-00000001-QINU

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 [[Image:T--Linkoping_Sweden--CBD-sfGFPstorodl4.jpeg|300px|thumb|left|<b><i>Figure 1.</i></b> 1 liter growth medium containing CBD-sfGFP BL21 (DE3) (induced?) on a UV-table. ]]
+<br> Pictures above shows induced BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP. The picture to the left is a 1 liter growth medium with E.coli BL21 expressing CBD-sfGFP. The picture in the middle is a depiction of an induction gradient with BL21 and varying IPTG concentrations. The picture to the right is the same but with Vibrio natriegens Vmax instead. The induction gradient for Vibrio is more correlated with the IPTG concentration then E.Coli suggesting that the induction windows are different between the bacteria with CBD-sfGFP.
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