Difference between revisions of "Part:BBa K112100:Design"
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<partinfo>BBa_K112100 short</partinfo> | <partinfo>BBa_K112100 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. | This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. | ||
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PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI/DpnI) | PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI/DpnI) | ||
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mv002 Reverse Biobricking of <Cre! | mv002 Reverse Biobricking of <Cre! | ||
cgttaGGATCCttaatcgccatcttccagc | cgttaGGATCCttaatcgccatcttccagc | ||
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===Source=== | ===Source=== | ||
Revision as of 02:28, 15 August 2008
Cre enzyme without start codon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 39
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.
PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112100
mv001 Forward Biobricking of <Cre! ccataGAATTCatgAGATCTtccaatttactgaccgtacacc mv002 Reverse Biobricking of <Cre! cgttaGGATCCttaatcgccatcttccagc
Source
PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112100